ساخت پپتید (Peptide Synthesis)

Terminal modification

AC

N-Terminal

For

N-Terminal

Suc

N-Terminal

CBZ

N-Terminal

Mercaptoacetyl

N-Terminal

Mercaptoprppionyl

N-Terminal

Pyr

N-Terminal

Amidation

C-Terminal

Ester

C-Terminal

Cysteamide

C-Terminal

Hydroxy modified peptide

Phosphorylation

Ser, Thr, Tyr

Sulfation

Tyr

Fatty acid

Myristic acid
(Myristoyl)

N-Terminal amine or C-Termial (via Lys side chain amine)

Stearic acid (Stearoyl)

N-Terminal amine or C-Termial (via Lys side chain amine)

Palmitic acid (Palmitoyl)

N-Terminal amine or C-Termial (via Lys side chain amine)

Disulfied Bond (S-S)

Single intra disulfide

Via Cys-Cys (S-S) cyclic form

Two intra disulfides

Via Cys-Cys (S-S) cyclic form

Inter disulfide

Cys-Cys (S-S) form

Amid Cyclic peptide

Head-to-Tail cyclic

Via N-Terminal amine with C-Terminal Carboxylic acid, Amide cyclic form

Side Chain

Via internal Amine(Lys or Dap side chain) with internal Carboxylic acid (Asp or Glu side chain) Amide cyclic form

Special amino acid

D-Form Amino acids

Any

Unusual amino acids (Aib, Cit, Nle, Nva, Orn, Nal etc.)

Any

Labelling

Biotin

N-Terminal amine or C-Termial (via Lys side chain amine)

FITC

(Abs/Em = 494/519 nm)

N-Terminal amine or C-Termial (via Lys side chain amine)

FAM

(Abs/Em = 494/518 nm)

N-Terminal amine or C-Termial (via Lys side chain amine). Quencher is Dabcyl.

Rhodamin B
(Abs/Em = 552/588 nm)

N-Terminal amine or C-Termial (via Lys side chain amine)

TAMRA

(Abs/Em = 555/580 nm)

N-Terminal amine or C-Termial (via Lys side chain amine)

Mca
(Abs/Em = 328/393 nm)

N-Terminal amine or C-Termial (via Lys side chain amine). Quencher is Dnp.

Dansyl 

(Abs/Em = 335/526 nm)

N-Terminal amine or C-Termial (via Lys side chain amine). Quencher is Dabsyl.

EDANS (Abs/Em = 341/471 nm)

Via intenal Glu side chain. Quencher is Dabcyl.

AMC (Abs/Em = 360/405 nm)

C-Terminal

Dabcyl (Abs = 453 nm)

N-Terminal amine or C-Termial (via Lys side chain amine)

Dnp (Abs = ۳۴۸ nm)

N-Terminal amine or C-Termial (via Lys side chain amine)